Lead in cereals and related products are at very low contamination levels, residents' average daily intake of lead from grains and products was low in Zhejiang Province, but measures are still needed to reduce lead in cereal products considering the adverse health effects of lead.Improving nutrient absorption in pepper has become a vital prerequisite for growth to produce a sustainable yield. In this study, transcriptome gene expression in pepper inoculated with two types of phosphate-solubilizing bacteria (PSB) and grown under low and high nutrient levels (LN and HN) was analyzed. Results showed that the root length increased when pepper was grown under LN; however, the root structure was intensively tight under HN. https://www.selleckchem.com/MEK.html Our data revealed that the roots preferred horizontal growth than longitudinal growth under HN. PSB strains 'M01' and 'N3' significantly (P less then 0.01) increased the P uptake by 70.44% and 98.20%, respectively, but decreased the Ca2+ content by 8.96% and 9.13%, respectively, compared with the control (L1). Although no remarkable difference was detected in the chlorophyll content, inoculation with the two PSB strains decreased the Fe3+ content in pepper under HN. The total clean sequenced data from samples ranged between 5,923,659,118 and 9,955,045,953 bp. Transcriptome profiling revealed 320 upregulated and 449 downregulated genes in L3 versus L1 and 468 upregulated and 532 downregulated genes in L4 versus L1. Gene ontology analysis revealed that the biological processes, including response to stress and secondary metabolic process, were involved. Several pathways were subordinate to glycosphingolipid biosynthesis and linoleic acid and nitrogen metabolisms. Analysis of the eukaryotic orthologous group function revealed that most differential genes were attributed to RNA processing and modification, transcription, and signal transduction. Our results provided new insights into the molecular mechanism related to nutrient uptake in pepper inoculated with PSBs.Nitrogen is one of the essential element required for plant growth and development. In plants, most of the nitrogen is stored in arginine. Hence, metabolism of arginine to urea by arginase and its further hydrolysis to ammonia by urease is involved in nitrogen recycling to meet the metabolic demands of growing plants. In this respect, plant arginases differ from that of animals. Animals excrete urea while plants recycle the urea. However, the studies on the biochemical and biophysical characteristics of plant arginase are limited when compared to animal arginase(s). In this review, the structural and biochemical characteristics of various plant arginases are discussed. Moreover, the significance of arginase in nitrogen recycling is explained and recent literature on function and activation of plant arginases in response to various environmental (biotic and abiotic) insults is also presented.Eventual protective action of calcium (Ca, 100 or 1000 μM) against cadmium (Cd, 10 or 100 μM) toxicity in common lichen Hypogymnia physodes after 24 h of exposure was studied. Total Cd reached 482 and 2801 μg/g DW in 10 and 100 μM Cd treatments while Ca content reached over 23 mg/g DW in 1000 μM Ca treatment. Ca suppressed Cd accumulation by 23 and 38% in total fraction and completely in absorbed fraction. Fluorescence microscopy of Cd and Ca ions revealed good correlation with quantitative data. Cd stimulated increase in ROS formation and lipid peroxidation as detected using fluorescent reagents and quantification of H2O2 while co-application of Ca suppressed these effects. Formation of nitric oxide was mainly affected by cadmium. Cd depleted amount of amino acids but proteins or phenols remained unaffected by Cd or Ca. On the contrary, sum of thiols, reduced glutathione and ascorbic acid were depleted by Cd but reversed mainly by higher Ca dose. Among organic acids, only Cd-induced depletion of citric acid content was reversed by Ca. Data indicate that ameliorative effect of Ca under Cd excess in lichens is comparable with effect in plants and metabolic responses in various life lineages are discussed.As people age, they spend more time in and around their domestic environments, within which opportunities for health-enhancing physical activity (PA) may be limited. We examined the associations of frequency of excursions from home with accelerometer-determined PA and the total and prolonged sedentary behavior (SB, sitting for ?30 min) among community-dwelling older adults.
Overall, 434 older adults (men, 57.1 %; age, 70-79 years) wore an accelerometer (HJA-350IT, Omron Healthcare) for 7 days. Time spent in SB, light-intensity PA (LPA), moderate-to-vigorous PA (MVPA), and prolonged SB bout (?30 min) were calculated. Frequencies of excursions from home were categorized as 0-3, 4-5, and 6-7 days/week. Associations of excursions with PA and SB were tested by analysis of covariance adjusted for sociodemographic characteristics.
Frequencies were 14.3 %, 31.1 %, and 54.6 % for excursions 0-3, 4-5, and 6-7 days/week, respectively. For men, less frequent excursions from home were significantly associated with less MVPA and more total and prolonged SB times (estimated means [minutes/day] MVPA, 32, 42, and 48; total SB, 589, 549, and 539; prolonged SB, 344, 288, and 265; respectively; p &lt; 0.05). LPA was not statistically significant (p = 0.09). For women, less frequent excursions were significantly associated with all PA outcomes (SB 536, 497, and 467; prolonged SB 260, 213, and 204; LPA 328, 363, and 379; MVPA 36, 39, and 54, respectively).
Among community-dwelling Japanese older adults, more frequent excursions from home were associated with more PA, less SB, and more-favorable SB patterns, suggesting a potential strategy for preventive-health initiatives.
Among community-dwelling Japanese older adults, more frequent excursions from home were associated with more PA, less SB, and more-favorable SB patterns, suggesting a potential strategy for preventive-health initiatives.Institutionalized older adults have higher risks of diseases and worse health-related quality of life (HRQoL) than noninstitutionalized.
To evaluate the influence of physical state, nutritional status and self-perceived general health and oral health on the quality of life of institutionalized older adults in two Brazilian cities.
A multicenter cross-sectional study was conducted in 17 homes for the aged of two Brazilian cities. Six trained researchers interviewed 344 older adults. The performance of activities of daily living (Katz scale) and frailty status (Fried scale) were applied to evaluate the physical state. The Mini Nutritional Assessment Short-Form (MNA-SF®) and the body composition were used to screening the nutritional status. A hand dynamometer was used to measure the dominant hand grip strength. The 12-Item Short Form Survey for self-perceived health (SF-12) was used to determine the HRQoL. A Likert scale was used to assess the general and oral health self-perception. Multiple Poisson regression model was applied to analyze the data (α = 0.